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His Tag 抗体

WB, IP, IF 宿主: 小鼠 Monoclonal 5I8 unconjugated
产品编号 ABIN7250827
发货至: 中国
  • 抗原 See all His Tag 抗体
    His Tag
    适用
    请咨询
    宿主
    • 88
    • 60
    • 11
    • 9
    • 5
    • 4
    • 1
    • 1
    小鼠
    克隆类型
    • 90
    • 76
    • 10
    单克隆
    标记
    • 76
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    This His Tag antibody is un-conjugated
    应用范围
    • 138
    • 89
    • 36
    • 25
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    • 16
    • 14
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    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
    产品特性
    Tag Antibody
    纯化方法
    Protein A purification
    免疫原
    Synthetic Peptide
    克隆位点
    5I8
    亚型
    IgG
    Top Product
    Discover our top product His Tag Primary Antibody
  • 应用备注
    WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Store at -20°C. Avoid freeze / thaw cycles.
  • 抗原
    His Tag
    别名
    His-Tag (His Tag 产品)
    物质类
    Tag
    背景
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
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