USP32 (ubiquitin specific peptidase 32), also known as NY-REN-60, is a 1,604 amino acid protein that contains one DUSP domain and three EF-hand calcium binding domains. Localized to membranes in a lipid-anchored fashion and expressed in all normal tissues, USP32 catalyzes the conversion of a ubiquitin C-terminal thioester to a free ubiquitin and a thiol, a reaction that may influence several cellular processes.