DYKDDDDK Tag 抗体
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- 抗原 See all DYKDDDDK Tag products
- DYKDDDDK Tag
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适用
- Various Species
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This DYKDDDDK Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)
- 原理
- Anti-FLAG Octapeptide (FLAG) Tag Monoclonal Antibody
- 特异性
- The antibody is a mouse monoclonal antibody raised against FLAG. It has been selected for its ability to recognize FLAG in immunohistochemical staining and western blotting.
- 纯化方法
- Protein A + Protein G affinity chromatography
- 免疫原
- OVA Conjugated FLAG Octapeptide (FLAG)
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- 应用备注
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Western blotting: 0.5-5 μg/mL Immunocytochemistry in formalin fixed cells/Immunofluorescence: 5-30 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- DYKDDDDK Tag
- 别名
- FLAG Octapeptide (DYKDDDDK Tag 产品)
- 物质类
- Tag
- 背景
- FLAG epitope, FLAG-Tag
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