PRAME 抗体 (AA 416-502)
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- 抗原 See all PRAME 抗体
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
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抗原表位
- AA 416-502
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PRAME antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 原理
- Polyclonal Antibody to Preferentially Expressed Antigen In Melanoma (PRAME)
- 特异性
- The antibody is a rabbit polyclonal antibody raised against PRAME. It has been selected for its ability to recognize PRAME in immunohistochemical staining and western blotting.
- 交叉反应
- Pig
- 纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- 免疫原
- Recombinant Preferentially Expressed Antigen In Melanoma (PRAME) corresdonding to Asn416~Leu502 (Accession # P78395)
- 亚型
- IgG
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- 应用备注
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Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- 别名
- Preferentially Expressed Antigen In Melanoma (PRAME 产品)
- 别名
- PRAME antibody, CT130 antibody, MAPE antibody, OIP-4 antibody, OIP4 antibody, 4930534P07Rik antibody, preferentially expressed antigen in melanoma antibody, PRAME antibody, Prame antibody
- 背景
- MAPE, OIP4, CT130, Cancer/Testis Antigen 130, Opa-interacting protein 4, Melanoma antigen preferentially expressed in tumors
- 途径
- Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
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