HLA-B 抗体 (AA 26-309)
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- 抗原 See all HLA-B 抗体
- HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))
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抗原表位
- AA 26-309
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HLA-B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 原理
- Polyclonal Antibody to Major Histocompatibility Complex Class I B (MHCB)
- 特异性
- The antibody is a rabbit polyclonal antibody raised against MHCB. It has been selected for its ability to recognize MHCB in immunohistochemical staining and western blotting.
- 交叉反应
- Pig
- 纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- 免疫原
- Recombinant Major Histocompatibility Complex Class I B (MHCB) corresdonding to Ser26~Val309 with N-terminal His Tag
- 亚型
- IgG
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- 应用备注
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Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))
- 别名
- Major Histocompatibility Complex Class I B (HLA-B 产品)
- 别名
- AS antibody, HLAB antibody, SPDA1 antibody, mhc1b antibody, major histocompatibility complex, class I, B antibody, major histocompatibility complex nonclassical class Ib L homeolog antibody, HLA-B antibody, BOLA-B antibody, MAMU-B18 antibody, DKFZP469N1225 antibody, mhc1b.L antibody
- 背景
- MHC-B, HLA-B, HLAB, AS, SPDA1, Leukocyte Antigen B, Ankylosing Spondylitis, HLA class I histocompatibility antigen, B-7 alpha chain
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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