Leptin 抗体 (AA 22-167)
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- 抗原 See all Leptin (LEP) 抗体
- Leptin (LEP)
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抗原表位
- AA 22-167
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Leptin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 原理
- Monoclonal Antibody to Leptin (LEP)
- 特异性
- The antibody is a mouse monoclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.
- 交叉反应
- 大鼠
- 纯化方法
- Protein A + Protein G affinity chromatography
- 免疫原
- Recombinant Leptin (LEP) corresdonding to Val22~Cys167 with N-terminal His Tag
- 克隆位点
- C1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product LEP Primary Antibody
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- 应用备注
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Western blotting: 0.5-2 μg/mL
1:500-2000 Immunohistochemistry: 5-20 μg/mL
1:50-200 Immunocytochemistry: 5-20 μg/mL
1:50-200 Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- Leptin (LEP)
- 别名
- Leptin (LEP 产品)
- 别名
- ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody
- 背景
- OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein
- 途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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