This SARS-CoV-2 NSP12 (RdRP) antibody is un-conjugated
应用范围
Western Blotting (WB), ELISA
纯化方法
SARS-CoV-2 (COVID-19) NSP12 antibody is affinity chromatography purified via peptide column.
免疫原
Anti-SARS-CoV-2 (COVID-19) NSP12 antibody was raised against a peptide corresponding to 16 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) NSP12 protein. The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) NSP12 protein.
Antibody validated: SARS-CoV-2 (COVID-19) NSP12 antibody can detect 2 ng of free peptide at 1 ?g/mL in ELISA. It can detect SARS-CoV-2 NSP12 recombinant protein by ELISA and WB. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
SARS-CoV-2 (COVID-19) NSP12 antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
SARS-CoV-2 (COVID-19) NSP12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Sun, Abriola, Niederer, Pedersen, Alfajaro, Silva Monteiro, Wilen, Ho, Gilbert, Surovtseva, Lindenbach, Guo: "Restriction of SARS-CoV-2 replication by targeting programmed -1 ribosomal frameshifting." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, Issue 26, (2021) (PubMed).
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP12 interacts with nsp7 and nsp8.