Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
特异性
IL-11 antibody is human specific. At least two isoforms of IL-11 are known to exist, this antibody will only detect the longest isoform of IL-11.
纯化方法
IL-11 Antibody is affinity chromatography purified via peptide column.
免疫原
IL-11 antibody was raised against a 16 amino acid peptide from near the amino terminus of human IL-11. The immunogen is located within amino acids 40 - 90 of IL-11.
IL11
适用: 小鼠, 大鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 350
应用备注
IL-11 antibody can be used for the detection of IL-11 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry at 10 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
IL-11 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
IL-11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Interleukin 11 is a pleiotropic cytokine that has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6 (1). It directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (2). IL-11 promotes the proliferation of hepatocytes in response to liver damage. IL-11 binds to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 (3). Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelo-suppressive chemotherapy (4).