HOOK3 antibody is human, mouse and rat reactive. HOOK3 antibody is predicted to not cross-react with HOOK1 and HOOK2.
纯化方法
HOOK3 Antibody is affinity chromatography purified via peptide column.
免疫原
HOOK3 antibody was raised against a 19 amino acid peptide from near the amino terminus of human HOOK3. The immunogen is located within amino acids 210 - 260 of HOOK3.
HOOK3
适用: 人, 小鼠
WB, IP
宿主: 兔
Polyclonal
unconjugated
应用备注
HOOK3 antibody can be used for the detection of HOOK3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry at 10 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
HOOK3 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
HOOK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
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背景
The HOOK proteins (HOOK1, HOOK2 and HOOK3) comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules mediating the spatial organization of diverse membrane-trafficking systems, their more divergent C-terminal domains mediate binding to organelles (1,2). HOOK3 exists as a homodimer and participates in defining the architecture and localization of the Golgi complex through its central coiled-coil domain (3). HOOK3 may regulate clearance of endocytosed receptors such as MSR1 (3,4).