Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
PMEPA1 antibody is human, mouse and rat reactive. At least four isoforms are known to exist, this antibody will detect all of the isoforms.
纯化方法
PMEPA1 antibody is affinity chromatography purified via peptide column.
免疫原
PMEPA1 antibody was raised against a 16 amino acid peptide near the center of human PMEPA1. The immunogen is located within amino acids 160 - 210 of PMEPA1.
PMEPA1 antibody can be used for detection of PMEPA1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
PMEPA1 antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
PMEPA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
The prostate transmembrane protein, androgen induced 1 (PMEPA1) protein is a transmembrane protein that contains a Smad interacting motif (SIM) (1,2). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins (3). Overexpression of this gene may play a role in multiple types of cancer (2,4).