Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
TAU antibody is human, mouse and rat reactive. Multiple isoforms of TAU are known to exist, this antibody will only detect the two longest isoforms.
纯化方法
TAU antibody is affinity chromatography purified via peptide column.
免疫原
TAU antibody was raised against a 19 amino acid peptide near the amino terminus of human TAU. The immunogen is located within amino acids 210 - 260 of TAU.
TAU antibody can be used for detection of TAU by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
TAU antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
TAU antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
DDPAC antibody, FTDP-17 antibody, MAPTL antibody, MSTD antibody, MTBT1 antibody, MTBT2 antibody, PPND antibody, TAU antibody, tau antibody, PHF-tau antibody, microtubule associated protein tau antibody, MAPT antibody
背景
The microtubial-associated protein TAU (MAPT), more commonly known as TAU, is is normally a highly soluble protein found predominantly in neurons (1), but accumulations of highly phosphorylated tau protein aggregates are observed in several neurodegenerative diseases including Alzheimer's disease, progressive supranuclear palsy, corticobasal degeneration, and frontotemporal lobar dementia. It was thought that these pathological tau aggregates were the toxic form of tau, but recent studies indicate that soluble and highly phosphorylated tau species are more closely associated with synaptic dysfunction and cell loss (2,3). Mutations in the TAU gene have also been associated with several of these neurodegenerative diseases (4).