RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist, this antibody will detect all three isoforms.
纯化方法
RHOT1 antibody is affinity chromatography purified via peptide column.
免疫原
RHOT1 antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOT1. The immunogen is located within amino acids 40 - 90 of RHOT1.
RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in rat samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
RHOT1 antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
RHOT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
抗原
RHOT1
(Ras Homolog Gene Family, Member T1 (RHOT1))
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背景
The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1). RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3). Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4).