Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
特异性
LIN54 antibody is human, mouse, and rat reactive. Multiple isoforms of LIN54 are known to exist.
纯化方法
LIN54 antibody is affinity chromatography purified via peptide column.
免疫原
LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54. The immunogen is located within the last 50 amino acids of LIN54.
LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
LIN54 antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
LIN54 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1). LIN54 is required for cell cycle progression and can bind to the cdc2 promoter in a sequence-specific manner (2).