BHLHA15 antibody is human, mouse and rat reactive.
纯化方法
BHLHA15 antibody is affinity chromatography purified via peptide column.
免疫原
BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15. The immunogen is located within amino acids 60 - 110 of BHLHA15.
BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in rat samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
BHLHA15 antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
BHLHA15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
抗原
BHLHA15
(Basic Helix-Loop-Helix Family, Member A15 (BHLHA15))
BHLHB8 antibody, MIST1 antibody, 1810009C13Rik antibody, Bhlhb8 antibody, MIST-1 antibody, Mist1 antibody, bHlH antibody, basic helix-loop-helix family member a15 antibody, basic helix-loop-helix family, member a15 antibody, BHLHA15 antibody, Bhlha15 antibody
背景
BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).