Western Blotting (WB), ELISA, Immunofluorescence (IF)
特异性
At least two isoforms are known to exist. This antibody will recognize both isoforms. POU3F2 antibody is predicted to not cross-react with other members of the POU domain class 3 family.
纯化方法
POU3F2 Antibody is affinity chromatography purified via peptide column.
免疫原
Rabbit polyclonal POU3F2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human POU3F2. The immunogen is located within the last 50 amino acids of POU3F2.
POU3F2 antibody can be used for detection of POU3F2 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
POU3F2 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
POU3F2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
BRN2 antibody, N-Oct3 antibody, OCT7 antibody, OTF-7 antibody, OTF7 antibody, POUF3 antibody, brn-2 antibody, oct-7 antibody, XlPOU3 antibody, pou3f2 antibody, pou3f2-A antibody, pou3f4 antibody, 9430075J19Rik antibody, A230098E07Rik antibody, Brn-2 antibody, Brn2 antibody, Otf7 antibody, XLPOU3b antibody, pou47 antibody, zp47 antibody, zp47pou antibody, brn-1.2 antibody, brn1.2 antibody, id:ibd5088 antibody, POU class 3 homeobox 2 antibody, POU class 3 homeobox 2 L homeolog antibody, POU domain, class 3, transcription factor 2 antibody, POU class 3 homeobox 2 S homeolog antibody, POU class 3 homeobox 2b antibody, POU class 3 homeobox 2a antibody, POU3F2 antibody, pou3f2.L antibody, Pou3f2 antibody, pou3f2.S antibody, pou3f2b antibody, pou3f2a antibody
背景
POU3F2 Antibody: POU3F2, also commonly known as brain-2, is a member of a family of POU domain genes expressed in mouse brain and is thought to be involved in the development of the neocortex and establishment of neural cell lineage. Recent studies suggest that POU3F2 may be involved with the development of neurodegenerative diseases as well as tumor development and proliferation. Along with the neural-lineage-specific transcription factors ASCL1 and MYT1L, POU3F2 can convert fibroblasts to functional neurons in vitro, a form of artificial stem cells termed induced neuronal (iN) cells, suggesting that these cells may be useful in the treatment of neurodegenerative diseases.