Western Blotting (WB), ELISA, Immunofluorescence (IF)
特异性
At least four isoforms of BCAR3 are known to exist, this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins.
纯化方法
BCAR3 Antibody is affinity chromatography purified via peptide column.
免疫原
BCAR3 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BCAR3. The immunogen is located within amino acids 30 - 80 of BCAR3.
BCAR3 antibody can be used for detection of BCAR3 by Western blot at 1 - 2 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
BCAR3 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
BCAR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
BCAR3
(Breast Cancer Anti-Estrogen Resistance 3 (BCAR3))
NSP2 antibody, SH2D3B antibody, AI131758 antibody, AND-34 antibody, BCAR3 antibody, breast cancer anti-estrogen resistance 3 antibody, breast cancer anti-estrogen resistance 3 L homeolog antibody, BCAR3 antibody, Bcar3 antibody, bcar3 antibody, bcar3.L antibody
背景
BCAR3 Antibody: Breast cancer anti-estrogen resistance 3 (BCAR3) was identified in the search for genes involved in the development of estrogen resistance. It contains a putative src homology 2 (SH2) domain and is partly homologous to the cell division cycle protein CDC48. BCAR3 is a binding partner with Cas, an adapter molecule that plays a role in cell proliferation, survival, cell adhesion, and mobility. BCAR3 functions synergistically with Cas to enhance Src activation and promote cell migration, and has been suggested to regulate Cas/Src association, Src kinase activity, and breast cancer adhesion signaling.