Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
特异性
ZNF521 antibody is human specific.
纯化方法
ZNF521 Antibody is affinity chromatography purified via peptide column.
免疫原
ZNF521 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human ZNF521. The immunogen is located within the first 50 amino acids of ZNF521.
ZNF521 antibody can be used for detection of ZNF521 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
ZNF521 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
ZNF521 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Znf521 antibody, EHZF antibody, Evi3 antibody, B930086A16Rik antibody, zinc finger protein 521 antibody, zinc finger protein 521 S homeolog antibody, Zfp521 antibody, ZNF521 antibody, znf521.S antibody
背景
ZNF521 Antibody: The zinc finger protein 521 (ZNF521) is a transcription factor containing an N-terminal transcriptional repressor motif and 30 zinc finger domains. It plays a role in both erythroid cell and osteoblast differentiation during development, inhibiting the activities of early B-cell factor 1 (EBF1) in erythroid cells and Runx2 in osteoblast precursors. ZFP521 binds to both Runx2 and histone deacetylase 3 (HDAC3), promotes their association and antagonizes Runx2 transcriptional activity in a HDAC3-dependent manner, thereby regulating osteoblast differentiation, skeletal development, and bone homeostasis.