ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
纯化方法
YPEL3 Antibody is affinity chromatography purified via peptide column.
免疫原
YPEL3 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human YPEL3. The immunogen is located within amino acids 20 - 70 of YPEL3.
YPEL3 antibody can be used for detection of YPEL3 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
YPEL3 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
YPEL3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
YPEL3 Antibody: YPEL3 (yippee-like 3) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL3 is a p53-regulated gene whose expression is induced by DNA damage and in turn induces cellular senescence. It appears to function as a tumor suppressor as it is downregulated in colon and breast tumors.