Western Blotting (WB), ELISA, Immunofluorescence (IF)
特异性
This antibody is specific for RKHD3 and will not recognize the other RKHD3 family of proteins.
纯化方法
RKHD3 Antibody is affinity chromatography purified via peptide column.
免疫原
RKHD3 antibody was raised against a 16 amino acid synthetic peptide from near the center of human RKHD3. The immunogen is located within amino acids 180 - 230 of RKHD3.
RKHD3 antibody can be used for detection of EPAC1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunofluorescence starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
RKHD3 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
RKHD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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背景
RKHD3 Antibody: Rkhd3, also known as MEX3B is a member of a novel family of four homologous human MEX3 proteins each containing two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one carboxy-terminal RING finger module. MEX3 proteins, including Rkhd3, are phosphoproteins that bind RNA through their KH domains and shuttle between the nucleus and the cytoplasm via the CRM1 export pathway. These proteins are a novel family of evolutionarily conserved RNA-binding proteins, differentially recruited to P bodies and potentially involved in post-transcriptional regulatory mechanisms. Rkhd3 is thought to act as a mechanism to fine-tune mRNA regulation in early Xenopus development, and with Rkhd4, but not Rkhd1, will co-localize with both the hDcp1a decapping factor and Argonaute (Ago) proteins in processing bodies (P bodies), recently characterized as centers of mRNA turnover.