ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
特异性
Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members.
纯化方法
PRICKLE4 Antibody is affinity chromatography purified via peptide column.
免疫原
PRICKLE4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PRICKLE4. The immunogen is located within the first 50 amino acids of PRICKLE4.
PRICKLE4 antibody can be used for detection of PRICKLE4 by Western blot at 0.25 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
PRICKLE4 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
PRICKLE4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PRICKLE4 Antibody: PRICKLE4, also known as over-expressed breast tumor protein (OBTP), is a member of the LIM family of proteins. It possesses two LIM domains as well as a PET domain and is thought to localize to the nucleus. Its expression in multiple malignant tissue differentiations indicate that it may play a role in cancer differentiation. Two other members of the LIM family, PRICKLE1 and PRICKLE2, are thought to function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity, suggesting the PRICKLE4 may play a similar role.