Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
特异性
At least two isoforms of SLC35D3 are known to exist, this antibody will recognize both isoforms. SLC35D3 antibody is predicted to not cross-react with SLC35D1 or SLC35D2.
纯化方法
SLC35D3 Antibody is affinity chromatography purified via peptide column.
免疫原
SLC35D3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SLC35D3. The immunogen is located within the last 50 amino acids of SLC35D3.
SLC35D3 antibody can be used for detection of SLC35D3 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
SLC35D3 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
SLC35D3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
SLC35D3
(Solute Carrier Family 35, Member D3 (SLC35D3))
FRCL1 antibody, bA55K22.3 antibody, 6230421J19Rik antibody, Frcl1 antibody, solute carrier family 35, member D3 antibody, solute carrier family 35 member D3 antibody, Slc35d3 antibody, SLC35D3 antibody, slc35d3 antibody
背景
SLC35D3 Antibody: The solute carrier family SLC35 consists of at least 17 proteins that act as nucleotide sugar transporters localized to the Golgi apparatus and endoplasmic reticulum. The novel protein SLC35D3 is highly homologous to SLC35D1 and SLC35D2, both of which transport UDP-glucuronic acid and UDP-N-acetylgalactosamine, suggesting that SLC35D3 is also involved in the transport of nucleotide sugars. It has been suggested that SLC35D3 regulates platelet dense granules, lysosome-related organelles which contain high concentrations of several biologically important low molecular weight molecules necessary for normal blood homeostasis.