ELISA, Western Blotting (WB), Immunofluorescence (IF)
特异性
At least three isoforms of ZMYM3 are known to exist, this antibody will detect all three. ZMYM3 antibody is predicted to not cross-react with other ZMYM protein family members.
纯化方法
ZMYM3 Antibody is affinity chromatography purified via peptide column.
免疫原
ZMYM3 antibody was raised against a 18 amino acid synthetic peptide near the center of human ZMYM3. The immunogen is located within amino acids 220 - 270 of ZMYM3.
ZMYM3 antibody can be used for detection of ZMYM3 by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
ZMYM3 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
ZMYM3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
ZMYM3 Antibody: Zinc-finger proteins contain DNA-binding domains characterized by the unique role of zinc and have a wide variety of functions such as transcriptional activation or repression. The protein folding and the DNA binding ability are governed by the coordination of a zinc ion. As a member of the MYM (myeloproliferative and mental retardation) gene family, ZMYM3 is highly conserved in vertebrates and most abundantly expressed in the brain. The encoded protein is a component of histone deacetylase-containing multiprotein complexes that function through modifying chromatin structure to keep genes silent. A chromosomal translocation (X,13) involving this gene is associated with X-linked mental retardation.