At least two isoforms of ZBTB8 are known to exist, this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.
纯化方法
ZBTB8 Antibody is affinity chromatography purified via peptide column.
免疫原
ZBTB8 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ZBTB8. The immunogen is located within the last 50 amino acids of ZBTB8.
ZBTB8A
适用: 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 647
应用备注
ZBTB8 antibody can be used for detection of ZBTB8 by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
ZBTB8 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
ZBTB8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
ZBTB8A
(Zinc Finger and BTB Domain Containing 8A (ZBTB8A))
ZBTB8 Antibody: The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. ZBTB8, also known as BOZ-F1, is present in multiple tissues, and is highly expressed in the cytoplasm of the placenta. While little is known about ZBTB8, other ZBTB proteins, such as ZBTB4 bind methylated DNA and repress transcription. Another ZBTB proteins, ZBTB7A, has been implicated as a proto-oncogene whose overexpression contributes to malignancy in breast cancer, suggesting that ZBTB8 may act as a transcriptional repressor or be involved in tumorigenesis.