Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
纯化方法
CaBP7 Antibody is affinity chromatography purified via peptide column.
免疫原
CaBP7 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CaBP7. The immunogen is located within the first 50 amino acids of CaBP7.
CaBP7 antibody can be used for detection of CaBP7 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 4 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
CaBP7 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
CaBP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CaBP7 Antibody: Calcium binding proteins (CaBP) play a crucial role in the calcium-mediated cellular signal transduction pathway in the central nervous system. The CaBP family shares much similarity with CaM I (calmodulin), and it has been shown that CaBP proteins can substitute functionally for, and possibly augment the function of, CaM I. CaBP7 (Calcium-binding protein 7), contains two EF-hand domains for calcium binding and shares 70 % homology with CaBP8 and 50 % homology with CaM I. It negatively regulates Golgi-to-plasma membrane trafficking by interacting with PI4KB and inhibiting its activity. CaBP7 and 8 possess a targeting mechanism that is unique amongst the CaBPs that may contribute to differential functional Ca2+-sensing by these family members.