ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
纯化方法
SCUBE1 Antibody is affinity chromatography purified via peptide column.
免疫原
SCUBE1 antibody was raised against a 14 amino acid synthetic peptide near the center of human SCUBE1. The immunogen is located within amino acids 460 - 510 of SCUBE1.
SCUBE1 antibody can be used for detection of SCUBE1 by Western blot at 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
SCUBE1 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
SCUBE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
7330410C13Rik antibody, A630023E24Rik antibody, signal peptide, CUB domain and EGF like domain containing 1 antibody, signal peptide, CUB domain, EGF-like 1 antibody, SCUBE1 antibody, Scube1 antibody
背景
SCUBE1 Antibody: SCUBE1 is an EGF-related protein with a CUB (C1s-like) domain that is expressed primarily in the developing gonad, nervous system, somites, surface ectoderm and limb buds. The N-terminal EGF-like repeats of SCUBE1 participate in the formation of adhesions in a Ca2+-dependent fashion. In contrast, the C-terminal cysteine-rich motifs and CUB domain can directly bind and antagonize bone morphogenic protein activity, genetic ablation of this region resulted in brain malformation in mice embryos, suggesting SCUBE1 plays a role in brain morphogenesis. SCUBE1 has also been implicated in early craniofacial development in mice.