Western Blotting (WB), ELISA, Immunofluorescence (IF)
纯化方法
APC7 Antibody is affinity chromatography purified via peptide column.
免疫原
APC7 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human APC7. The immunogen is located within amino acids 520 - 570 of APC7.
APC7 antibody can be used for detection of APC7 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunoflourescence starting at 20 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
APC7 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
APC7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
APC7 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC7 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC7 is required for proper protein ubiquitination function of APC/C and for the interaction of APC/C with various transcription coactivators.