Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
At least two isoforms of LRFN5 are known to exist. This antibody is predicted to not cross-react with other members of the LRFN family.
纯化方法
LRFN5 Antibody is affinity chromatography purified via peptide column.
免疫原
LRFN5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human LRFN5. The immunogen is located within amino acids 650 - 700 of LRFN5.
LRFN5
适用: 哺乳动物
IHC, ISt
宿主: 小鼠
Monoclonal
N140A-12
unconjugated
应用备注
LRFN5 antibody can be used for detection of LRFN5 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
LRFN5 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
LRFN5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
LRFN5
(Leucine Rich Repeat and Fibronectin Type III Domain Containing 5 (LRFN5))
C14orf146 antibody, FIGLER8 antibody, SALM5 antibody, AI427653 antibody, AI604817 antibody, C130061B21 antibody, mKIAA4208 antibody, leucine rich repeat and fibronectin type III domain containing 5 antibody, LRFN5 antibody, Lrfn5 antibody
背景
LRFN5 Antibody: LRFN5 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while LRFN2 and LRFN5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system.