Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
纯化方法
MADD Antibody is Antibody is DEAE purified.
免疫原
MADD antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human MADD. The immunogen is located within the last 50 amino acids of MADD.
MADD
适用: 小鼠
IF (p), IF (cc)
宿主: 兔
Polyclonal
AbBy Fluor® 555
应用备注
MADD antibody can be used for detection of MADD by Western blot at 1 - 2 mg/mL. 200 to 220 kDa bands should be detected. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
MADD Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
MADD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
MADD antibody, MGC145371 antibody, DENN antibody, IG20 antibody, RAB3GEP antibody, 9630059K23Rik antibody, MAP kinase activating death domain antibody, MAP-kinase activating death domain antibody, MADD antibody, madd antibody, Madd antibody
背景
MADD Antibody: MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98 % identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greater than 94 % overall identity to a GDP/GTP exchange protein Rab3-GEP. MADD is 87 % identical to KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.