Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
纯化方法
GFR alpha 2 Antibody is Ion exchange chromatography purified.
免疫原
GFR alpha 2 antibody was raised against a 15 amino acid peptide near the center of human GFR alpha 2. The immunogen is located within amino acids 360 - 410 of GFR alpha 2.
GFR alpha 2 antibody can be used for detection of GFR alpha 2 by Western blot at 1 μ,g/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
GFR alpha 2 Antibody is supplied in PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C,4 °C
储存方法
GFR alpha 2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
GFR alpha 2 Antibody: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNRalpha, which is a cell surface receptor for NTN. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently. NTNRalpha was also termed GDNFRβ, Ret ligand 2 (RETL2) or TGFbeta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα,-2 recently. GFRα,-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRα,-2, and the Ret PTK form a complex to transduce NTN signal and to mediate NTN function.