In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. In addition, TET proteins oxidize 5- methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5- formylcytosine (5fC), and 5-carboxylcytosine (5caC). TDG / Thymine-DNA glycosylase functions to correct G/T mispairs to G/C pairs and plays a role in active DNA demethylation by recognizing 5fC and 5caC and converting these to cystosine through the base excision repair (BER) pathway. TDG / Thymine-DNA glycosylase antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Mouse samples.
纯化方法
Protein A Chromatography
免疫原
This antibody was raised against a full-length recombinant protein corresponding to mouse thymine DNA glycosylase (TDG).
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.