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Rpb1 CTD 抗体 (pSer2)

适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 3E7C7 unconjugated
产品编号 ABIN6972624
发货至: 中国
  • 抗原 See all Rpb1 CTD products
    Rpb1 CTD (RNA Polymerase II CTD)
    抗原表位
    • 9
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    pSer2
    适用
    • 23
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 10
    • 9
    • 4
    大鼠
    克隆类型
    • 14
    • 9
    单克隆
    标记
    • 23
    This Rpb1 CTD antibody is un-conjugated
    应用范围
    • 23
    • 17
    • 14
    • 10
    • 7
    • 4
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    产品特性
    RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation. RNA pol II CTD phospho Ser2 antibody (mAb) (Clone 3E7C7) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.
    纯化方法
    Protein G Chromatography
    免疫原
    This antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 2.
    克隆位点
    3E7C7
    亚型
    IgG2a
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 缓冲液
    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • 抗原
    Rpb1 CTD (RNA Polymerase II CTD)
    别名
    RNA pol II CTD (Rpb1 CTD 产品)
    分子量
    240 kDa
    NCBI登录号
    NP_000928
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