OGT 抗体 (N-Term)
Quick Overview for OGT 抗体 (N-Term) (ABIN6972463)
抗原
See all OGT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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产品特性
- OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression. OGT/O-GlcNAc transferase antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot, it has been shown to react with Human and Mouse samples.
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纯化方法
- Affinity Purified
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免疫原
- This antibody was raised against a recombinant protein corresponding to the N-terminal region of human OGT.
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亚型
- IgG
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anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (Internal Region) antibody
Verified OGT 适用: 人, 大鼠 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (AA 857-1036) antibodyOGT 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibodyOGT 适用: 人 WB, IF, IHC (p), IP, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibodyOGT 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibodyOGT 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibodyOGT 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (AA 606-1022) antibodyOGT 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibodyOGT 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 8G7 unconjugated Recombinant Antibody
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) (N-Term) antibodyOGT 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT) antibodyOGT 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))
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别名
- OGT/O-GlcNAc transferase
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分子量
- 120 kDa
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NCBI登录号
- NP_858058
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途径
- Regulation of Carbohydrate Metabolic Process
抗原
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