Western Blotting (WB), ChIP DNA-Sequencing (ChIP-seq)
产品特性
N-Myc (MYCN) is a member of the Myc family of proto-oncogenes. Myc family members play crucial roles in regulating cell proliferation, size and differentiation. N-Myc is required for proper development of the nervous system. Myc family proteins can induce malignant transformation inappropriately modulating gene transcription, leading to unchecked cell proliferation. Misexpression of N-Myc is often associated with pediatric neural cancers such as neuroblastoma, medulloblastoma and retinoblastoma. N-Myc antibody (pAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq and Western blot, it has been shown to react with Human samples.
纯化方法
Affinity Purified
免疫原
This N-Myc antibody was raised against a peptide derived from the C-terminal region of human N-Myc.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.