Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Supershift Assay (SSA)
产品特性
MAZ (Myc-associated zinc finger protein) functions as a transcription factor roles in both transcription initiation and termination. MAZ binds to two sites within the c-Myc promoter, ME1a1 and ME1a2. It also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors. MAZ also participates in skeletal muscle development through the activation of several skeletal muscle-specific genes. MAZ has also been reported to drive tumor-specific expression of PPAR-gamma in breast cancer cells. MAZ antibody (mAb) (Clone 133) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Supershift and Western blot, it has been shown to react with Human samples.
纯化方法
Protein G Chromatography
免疫原
This MAZ antibody was raised against recombinant human MAZ protein.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
抗原
MAZ
(MYC-Associated Zinc Finger Protein (Purine-Binding Transcription Factor) (MAZ))