KLF5 (Kruppel-like factor 5, IKLF, Basic transcription element-binding protein 2, BTEB2) is a zinc-finger transcription factor that plays a role in cell proliferation, differentiation, cell cycle regulation, angiogenesis, and carcinogenesis and is especially important in epithelial cells. KLF5 (along with KLF2 and KLF4) are highly expressed in undifferentiated mouse embryonic stem cells and downregulated during early stages of differentiation. KLF5 and KLF2 can substitute for KLF4 in the reprogramming of differentiated cells into stem cells. KLF5 has a pro-proliferative role in cells and loss of both alleles of KLF5 is embryonic lethal. KLF5 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.
纯化方法
Affinity Purified
免疫原
This KLF5 antibody was raised against a peptide derived from the N-terminus of mouse KLF5 (Krueppel-like factor 5).
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.