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Histone 3 抗体 (3meLys9)

H3 适用: 人 WB, IF, ChIP, ICC, DB, CUT&Tag 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN6972043
发货至: 中国
  • 抗原 See all Histone 3 (H3) 抗体
    Histone 3 (H3)
    抗原表位
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    3meLys9
    适用
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    宿主
    • 1203
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    克隆类型
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    多克隆
    标记
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    This Histone 3 antibody is un-conjugated
    应用范围
    • 1034
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    Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), Cleavage Under Targets and Tagmentation (CUT&Tag)
    产品特性
    Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression. Histone H3K9me3 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, CUT&Tag, Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
    纯化方法
    Protein A Chromatography
    免疫原
    This Histone H3 trimethyl Lys9 antibody was raised against a peptide including trimethyl-lysine 9 of histone H3.
    亚型
    IgG
    Top Product
    Discover our top product H3 Primary Antibody
  • 应用备注
    Recommended antibody dilutions for different applications:
    ChIP: 10 µg for each ChIP
    ICC/IF: 1 µg/mL dilution
    WB: 0.5 - 2 µg/mL dilution
    CUT&Tag: 1 µg for each 50 µL reaction
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 缓冲液
    Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
  • 抗原
    Histone 3 (H3)
    别名
    Histone H3 (H3 产品)
    别名
    H-3 antibody, histocompatibility 3 antibody, H3 antibody
    分子量
    17 kDa
    NCBI登录号
    NP_003522
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