Histone H3.1 抗体 (pSer28)
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- 抗原 See all Histone H3.1 (HIST1H3B) 抗体
- Histone H3.1 (HIST1H3B)
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抗原表位
- pSer28
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适用
- 人
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This Histone H3.1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 产品特性
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. There are three protein variants of Histone H3, Histone H3.1, 3.2 and 3.3. The incorporation of Histone H3.1 and H3.2 into nucleosomes is replication dependent, in contrast to Histone H3.3, which is independent of DNA synthesis and occurs throughout the cell cycle. Human Histone H3.1 and H3.3 are identical in amino acid sequences except at position 110 where H3.1 has a cysteine and H3.3 has a serine. Phosphorylation of serine 28 occurs in early mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells. Histone H3.1S28ph antibody (mAb) (Clone 5D10D4) was raised in a Rat host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
- 纯化方法
- Protein G Chromatography
- 免疫原
- This antibody was raised against a peptide corresponding to phospho-serine 28 of human Histone H3.1. This antibody does not react with Histone H3.3 phosphorylated on serine 28.
- 克隆位点
- 5D10D4
- 亚型
- IgG2b
- Top Product
- Discover our top product HIST1H3B Primary Antibody
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anti-Histone H3.1 (HIST1H3B) (pSer10) antibody
HIST1H3B 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (AA 21-39) antibodyHIST1H3B 适用: 人 WB, IF, ChIP, ICC, ChIP-seq 宿主: 小鼠 Monoclonal 1D4F2 unconjugated
anti-Histone H3.1 (HIST1H3B) (pSer28), (Ser28) antibodyHIST1H3B 适用: 人 WB, IF, ICC 宿主: 大鼠 Monoclonal 5D10D4 unconjugated
anti-Histone H3.1 (HIST1H3B) (AA 1-50), (pSer10) antibodyHIST1H3B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (Ser10) antibodyHIST1H3B 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Histone H3.1 (HIST1H3B) (pSer28) antibodyHIST1H3B 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- 抗原
- Histone H3.1 (HIST1H3B)
- 别名
- Histone H3.1 (HIST1H3B 产品)
- 别名
- H3 histone antibody, Hist1h2ai antibody, histone cluster 1, H2ai-like1 antibody, histone H3.1 antibody, Hht21p antibody, Hist1h2ail1 antibody, H3.1 antibody, HHT21 antibody
- 分子量
- 17 kDa
- NCBI登录号
- NP_003522
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