Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
产品特性
HIRA (DGCR1, TUP1) is a histone chaperone that preferentially places the variant Histone H3.3 in nucleosomes, replacing canonical Histone H3 during the cell cycle. HIRA is required for the periodic repression of histone gene transcription during the cell cycle and when complexed with ASF1a is involved in the formation of the senescence-associated heterochromatin foci. HIRA antibody (mAb) (Clone WC119.2H11) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human and Mouse samples.
纯化方法
Protein G Chromatography
免疫原
This HIRA antibody was raised against a GST-fusion protein containing residues 421-729 of human HIRA.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
抗原
HIRA
(HIR Histone Cell Cycle Regulation Defective Homolog A (HIRA))