Drosha is an RNAse III enzyme that is involved in the process of generating microRNA (miRNA). miRNA interacts with the RNA-induced silencing complex (RISC) to cleave mRNA and thus downregulate gene expression. Drosha is a double stranded RNAse and part of the Microprocessor complex. Other RNAse III enzymes include Dicer and Argonaute. Drosha initiates processing of the miRNA precursor (pri-miRNA) in the nucleus, which is then exported to the cytoplasm and further cleaved by Dicer into a mature miRNA. Drosha antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot, it has been shown to react with Human samples.
纯化方法
Affinity Purified
免疫原
This Drosha antibody was raised against a peptide within the C-terminal region of human Drosha.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.