CUL4B (Cullin 4B) is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, Histone H2A, Histone H3 and Histone H4 in response to radiation-induced DNA damage and may act as a transcriptional corepressor by ubiquitination of Histone H2AK119 and recruiting PRC2 complexes. CUL4B antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot, it has been shown to react with Human samples.
纯化方法
Affinity Purified
免疫原
This antibody was raised against a peptide within the N-terminal region of human CUL4B.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.