Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Dot Blot (DB)
产品特性
CENP-A (Centromere Protein A) is a Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. Recently, phosphorylation of CENP-A on Ser16 and Ser18 was reported and found to be important for chromosome segregation during mitosis. CENP-A phospho Ser18 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
纯化方法
Affinity Purified
免疫原
This antibody was raised against a synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.