Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)
产品特性
CAF-1 p60 (Chromatin assembly factor-1 p60 subunit, CAF1B, CHAF1B) is a subunit of the CAF-1 complex that is required for the assembly of nucleosomes onto newly replicated DNA. Specifically, CAF-1 assists in the loading of the H3-H4 tetramer and may be involved in DNA damage repair. CAF-1 p60 is a WD-repeat containing protein related to HIRA. CAF-1 p60 antibody (mAb) (Clone 24) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot, it has been shown to react with Human and Mouse samples.
纯化方法
Protein G Chromatography
免疫原
The CAF-1 p60 antobody was raised against full-length CAF-1 purified from human 293 cells.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
抗原
CHAF1B
(Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))