Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)
产品特性
AIB1 (Amplified in Breast Cancer 1, Steroid Receptor Coactivator-3, Nuclear Receptor Coactivator 3, SRC-3, NCOA3) is a chromatin-associated protein that interacts with nuclear hormone receptors to modify their activity as transcriptional activators. AIB1 has histone acetyltransferase (HAT) activity and recruits p300/CBP-associated factor (PCAF) and CREB binding protein (CBP) as part of a multisubunit co-activator complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. It is Involved in the coactivation of different nuclear receptors, such as GR, ER, RARs, RXRs, TRs, VDR and PPARs. It is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFkB1 protein. AIB1 / SRC-3 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation and Western blot, it has been shown to react with Human samples.
纯化方法
Affinity Purified
免疫原
This AIB1 antibody was raised against a peptide derived from human AIB1/SRC-3.
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
缓冲液
Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.