电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

IL-12/IL-23 p40 抗体

适用: 人 Neut 宿主: 小鼠 Monoclonal MT3279H unconjugated
产品编号 ABIN6964021
发货至: 中国
  • 抗原 See all IL-12/IL-23 p40 products
    IL-12/IL-23 p40
    适用
    • 12
    • 2
    • 1
    宿主
    • 12
    • 2
    小鼠
    克隆类型
    • 14
    单克隆
    标记
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL-12/IL-23 p40 antibody is un-conjugated
    应用范围
    • 9
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Neutralization (Neut)
    特异性
    Native and recombinant p40 subunit of human IL-12 and IL-23
    纯化方法
    Purified from in vitro cultures by protein G affinity chromatography.
    免疫原
    Recombinant human IL-23
    克隆位点
    MT3279H
    亚型
    IgG2b
  • 应用备注
    Neutralization of human IL-12 and IL-23 bioactivity. It is recommended to establish the optimal antibody concentration for the assay system used. Neutralization Neutralization assay: Neutralization of human IL-12 and human IL-23 induced IFN-γ production (see graphs). To determine the neutralizing capacity and ND50 on IL-12 induced IFN-γ production, mixtures of various concentrations of mAb MT3279H, human recombinant IL-12 (10 ng/mL) and recombinant IL-1β (50 ng/mL) were added to human PBMC. For determination of the neutralizing capacity and ND50 on IL-23 induced IFN-γ production, mixtures of various concentrations of mAb MT3279H, human recombinant IL-23 (10 ng/ ml) and recombinant IL-18 (50 ng/mL) were added to human PBMC. Following overnight incubation, cell supernatants were quantified in IFN-γ ELISA. Neutralization dose 50 (ND50): MT3279H neutralized the effect of IL-12 and IL-23 at a ND50value of 0.2-0.5 μg/mL when 10 ng/mL of recombinant human IL-12 or IL-23 was present. ND50 is defined as the concentration of antibody that results in 50 % inhibition of the biological response under the experimental conditions specified.
    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    supplied at 1 mg/mL in PBS free of preservatives
    储存液
    Without preservative
    储存条件
    4 °C,-20 °C
    储存方法
    Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
    有效期
    18 months
  • Saghafian-Hedengren, Sohlberg, Theorell, Carvalho-Queiroz, Nagy, Persson, Nilsson, Bryceson, Sverremark-Ekström: "Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells." in: Journal of virology, Vol. 87, Issue 24, pp. 13446-55, (2014) (PubMed).

  • 抗原
    IL-12/IL-23 p40
    别名
    IL12/23 (p40) (IL-12/IL-23 p40 产品)
    基因ID
    3592, 3593
You are here:
客服