IL-4 抗体 (PromoFluor-488 Premium)
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- 抗原 See all IL-4 (IL4) 抗体
- IL-4 (IL4) (Interleukin 4 (IL4))
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适用
- 人, Non-Human Primate
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL-4 antibody is conjugated to PromoFluor-488 Premium
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应用范围
- Flow Cytometry (FACS)
- 特异性
- Native IL-4
- 交叉反应 (详细)
- The monoclonal antibody cross-reacts with IL-4 from non-human primates.
- 纯化方法
- Purified from in vitro cultures by protein G affinity chromatography.
- 免疫原
- Recombinant human IL-4
- 克隆位点
- IL4-3
- 亚型
- IgG1
- Top Product
- Discover our top product IL4 Primary Antibody
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- 应用备注
- Detection of IL-4 producing cells by flow cytometry. 5 μL is recommended for staining of 1 million cells in a total volume of 50 μL. Detection of IL-4 by flow cytometry in viable human PBMC. Cells were stimulated for 16 hours in the presence of PMA/ionomycin and Brefeldin A. Cells were stained with anti-CD3-BV421, fixed and permeabilized using 4 % paraformaldehyde and saponin, and further stained with PF488P-conjugated mAb IL4-3 (right panel). Non-stimulated cells shown as reference (left panel). The dot plot derives from gated events of typical lymphocyte characteristics in forward and side light scatter. Flow cytometry was performed on a BD FACSVerse system.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- in PBS with 0.2 % BSA and 0.09 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/thawing.
- 有效期
- 18 months
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- 抗原
- IL-4 (IL4) (Interleukin 4 (IL4))
- 别名
- IL4 (IL4 产品)
- 别名
- IL4 antibody, IL-4 antibody, BCGF-1 antibody, BCGF1 antibody, BSF-1 antibody, BSF1 antibody, Il-4 antibody, Il4e12 antibody, IL2 antibody, interleukin 4 antibody, IL4 antibody, Il4 antibody
- 基因ID
- 3565
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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