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- 抗原 See all CD45.2 products
- CD45.2 (CD45 (CD45.2 Allogen))
- 适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD45.2 antibody is conjugated to violetFluor™ 500
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应用范围
- Flow Cytometry (FACS)
- 纯化方法
- This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
- 克隆位点
- 104
- 亚型
- IgG2a kappa
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anti-CD45 (CD45.2 Allogen) antibody (PE)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 104 PE
anti-CD45 (CD45.2 Allogen) antibody (FITC)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 104 FITC
anti-CD45 (CD45.2 Allogen) antibody适用: 小鼠 FACS, IHC, IHC (fro) 宿主: 小鼠 Monoclonal 104 unconjugated
anti-CD45 (CD45.2 Allogen) antibody (Biotin)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 104 Biotin
anti-CD45 (CD45.2 Allogen) antibody (redFluor™ 710)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 104 redFluor™ 710
anti-CD45 (CD45.2 Allogen) antibody (violetFluor™ 450)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 104 violetFluor™ 450
anti-CD45 (CD45.2 Allogen) antibody (PerCP-Cy5.5)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 104 PerCP-Cy5.5
anti-CD45 (CD45.2 Allogen) antibody (APC-Cy7)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 104 APC-Cy7
anti-CD45 (CD45.2 Allogen) antibody (PE-Cy7)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 104 PE-Cy7
anti-CD45 (CD45.2 Allogen) antibody (APC)适用: 小鼠 FACS, IF 宿主: 小鼠 Monoclonal Ks20-8 APC
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- 应用备注
- This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system. violetFluor™ 500 dye is excited by the violet (405 nm) laser and has a peak emission of 500 nm. The recommended band pass filter for this dye is 525/20. violetFluor™ 500 can be used as an alternative for BD Horizon™ V500 or Pacific Orange®.
- 说明
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0.2 mg/mL
- 限制
- 仅限研究用
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- 缓冲液
- 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- 2-8°C protected from light
- 有效期
- 12 months
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- 抗原
- CD45.2 (CD45 (CD45.2 Allogen))
- 别名
- CD45.2 (CD45.2 产品)
- 背景
- The 104 antibody reacts with mouse CD45.2, also known as Ly5.2, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The 104 antibody is typically used as a leukocyte marker in Ly5.2 mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. The antibody has been demonstrated to be specific for CD45.2 and is not cross- reactive with CD45.1-bearing cells.
- 基因ID
- 19264
- UniProt
- P06800
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