MHC II (I-A/I-E) 抗体 (violetFluor™ 450)
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- 抗原 See all MHC II (I-A/I-E) products
- MHC II (I-A/I-E)
- 适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This MHC II (I-A/I-E) antibody is conjugated to violetFluor™ 450
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应用范围
- Flow Cytometry (FACS)
- 纯化方法
- This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
- 克隆位点
- M5-114-15-2
- 亚型
- IgG2b kappa
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anti-MHC II (I-A/I-E) antibody (FITC)
适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 FITC
anti-MHC II (I-A/I-E) antibody (Biotin)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 Biotin
anti-MHC II (I-A/I-E) antibody (APC)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 APC
anti-MHC II (I-A/I-E) antibody (violetFluor™ 500)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 violetFluor™ 500
anti-MHC II (I-A/I-E) antibody (PE-Cy7)适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114-15-2 PE-Cy7
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- 应用备注
- This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indi- cated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. violetFluor™ 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor™ 450 can be used as an alternative for Pacific Blue®, BD Horizon™ V450 or eFluor® 450.
- 说明
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0.2 mg/mL
- 限制
- 仅限研究用
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- 缓冲液
- 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- 2-8°C protected from light
- 有效期
- 12 months
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- 抗原
- MHC II (I-A/I-E)
- 别名
- MHC Class II (I-A/I-E) (MHC II (I-A/I-E) 产品)
- 背景
- The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g.The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.
- 基因ID
- 14961, 14969
- UniProt
- P14483
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