Recombinant Blue Fluorescent Protein 抗体 (AZDye 568)
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- 抗原 See all Blue Fluorescent Protein (BFP) products
- Blue Fluorescent Protein (BFP)
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
- 适用
- Entacmaea quadricolor
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宿主
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Alpaca
- Expression System
- E.coli
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克隆类型
- 单克隆
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标记
- This Blue Fluorescent Protein antibody is conjugated to AZDye 568
- 应用范围
- Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 原理
- Camelid sdAb anti-TagBFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clone 1H7
- 特异性
- Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor
- 交叉反应 （详细）
- Does not cross-react with common GFP- or dsRed derivatives.
- 产品特性
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- 纯化方法
- Produced in: E.coli
- 克隆位点
- 1H7
- Labeling Ratio
- a single fluorophore is coupled to exactly one sdAb
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anti-Blue Fluorescent Protein (BFP) antibody (Atto 488)
BFP 适用: Entacmaea quadricolor FACS, ICC, IF 宿主: Alpaca Monoclonal 1H7 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
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- 应用备注
- Recommended dilution 1:500
- 说明
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Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- The sdAb clone was lyophilized from PBS pH 7.4 containing 2% BSA. Please reconstitute with 200 µL of 50 % glycerol in deionized water. It is recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted in 200 µl, the concentration of single-domain antibody is 5 µM.
- 浓度
- 5 μM
- 缓冲液
- 5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light!
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. It is recommend storing the reconstituted protein at 4 °C.
- 有效期
- 12 months
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- 抗原
- Blue Fluorescent Protein (BFP)
- 别名
- TagBFP (BFP 产品)
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