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Recombinant SARS-CoV-2 Spike S1 抗体 (RBD)

Name: Recombinant SARS-CoV-2 Spike S1 抗体 (RBD)
SKU: ABIN6952616
Availability: OutOfStock

适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, Neut 宿主: Human Monoclonal unconjugated Recombinant Antibody

ELISA

Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (ABIN6952616) was detected by SARS-CoV-2 Inhibitor screening Kit (ABIN6952717) with a half maximal inhibitory concentration (IC50) of 1. (Recombinant SARS-CoV-2 Spike S1 抗体 (RBD))

ELISA

Immobilized SARS-CoV-2 S1 protein, His Tag (ABIN6952623) at 2 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (ABIN6952616) with a linear range of 0. (Recombinant SARS-CoV-2 Spike S1 抗体 (RBD))

ELISA

Anti-SARS-CoV-2 RBD Neutralizing Antibody (ABIN6952616), Human IgG1 on SDS-PAGE under reducing (R) condition. (Recombinant SARS-CoV-2 Spike S1 抗体 (RBD))

10 images

(7 references)

产品编号 ABIN6952616

发货至: 中国

Datasheet as PDF

抗原 See all SARS-CoV-2 Spike S1 抗体

SARS-CoV-2 Spike S1

抗体类型

Recombinant Antibody

生物活性

Active
抗原表位
12

3

2

2

1
RBD
适用
40

7

4

3

3

3

2

2

1

1
SARS Coronavirus-2 (SARS-CoV-2)
宿主
15

10

5

3

2

2

2

1

1
Human

Expression System

HEK-293 Cells
克隆类型
27

9

5
单克隆
标记
35

3

1

1

1
This SARS-CoV-2 Spike S1 antibody is un-conjugated
应用范围
40

9

8

7

5

5

4

4

3

3

2

1

1

1

1

1

1

1
ELISA, Neutralization (Neut)

原理

Anti-SARS-CoV-2 neutralizing antibody (Human IgG1)

特异性

The product is specific for SARS-CoV-2 Spike Protein S1 subunit and its RBD domain, and can inhibit the interaction between SARS-CoV-2 Spike Protein RBD and ACE2.

交叉反应（详细）

This product is a specific neutralizing antibody against SARS-CoV-2 Spike Protein RBD domain and no cross-reactivity was detected against SARS-CoV Spike protein. The cross-reactivity with other coronaviruses has not been tested yet.

产品特性

Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 recognizes the SARS-CoV-2 Spike Protein RBD domain and can inhibit the interaction between SARS-CoV-2 Spike Protein RBD and ACE2. It has not been tested with other coronovirus. It is expressed from human 293 cells (HEK293).

纯度

> 95 % as determined by SDS-PAGE.

过滤

0.22 μm filtered

亚型

IgG1

Biological Activity Comment

IC50 = 1.47 μg/mL using SARS-CoV-2 Inhibitor screening Kit

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适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV), SARS CoV-2 Alpha, SARS CoV-2 Epsilon, SARS CoV-2 Gamma, SARS CoV-2 Beta, SARS CoV-2 Eta, SARS CoV-2 Kappa, SARS CoV-2 Omicron, SARS CoV-2 Delta ELISA, IF, Crys, SPR, mIHC 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody

anti-SARS-CoV-2 Spike S1 (RBD) antibody
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anti-SARS-CoV-2 Spike S1 antibody
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anti-SARS-CoV-2 Spike S1 (RBD) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: 兔 Polyclonal unconjugated

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anti-SARS-CoV-2 Spike S1 (RBD) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, IF, Crys, SPR 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Lyophilized

缓冲液

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 . Normally trehalose is added as protectant before lyophilization.

储存条件

4 °C,-20 °C,-80 °C

储存方法

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.
Wu, Wu, Wang, Liu, Chu, Jiang, Kwong, Chow, Li, Chen: "Microfluidic particle dam for direct visualization of SARS-CoV-2 antibody levels in COVID-19 vaccinees." in: Science advances, Vol. 8, Issue 22, pp. eabn6064, (2022) (PubMed).

Chouchane, Grivel, Farag, Pavlovski, Maacha, Sathappan, Al-Romaihi, Abuaqel, Ata, Ismail Chouchane, Remadi, Halabi, Rafii, Al-Thani, Marr, Subramanian, Shan: "Dromedary camels as a natural source of neutralizing nanobodies against SARS-CoV-2." in: JCI insight, (2021) (PubMed).

Munitz, Edry-Botzer, Itan, Tur-Kaspa, Dicker, Marcoviciu, Goren, Mor, Lev, Gottesman, Muhsen, Cohen, Stein, Qimron, Freund, Wine, Gerlic: "Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature." in: Scientific reports, Vol. 11, Issue 1, pp. 3461, (2021) (PubMed).

Apostolou, Kyritsi, Vontas, Loizou, Hadjilouka, Speletas, Mouchtouri, Hadjichristodoulou: "Development and performance characteristics evaluation of a new Bioelectric Recognition Assay (BERA) method for rapid Sars-CoV-2 detection in clinical samples." in: Journal of virological methods, Vol. 293, pp. 114166, (2021) (PubMed).

Leach, Miller, Bentley, Mattiuzzo, Thomas, McAndrew, Van Montfort, Rabbitts: "Implementing a method for engineering multivalency to substantially enhance binding of clinical trial anti-SARS-CoV-2 antibodies to wildtype spike and variants of concern proteins." in: Scientific reports, Vol. 11, Issue 1, pp. 10475, (2021) (PubMed).

Park, Bazzill, Son, Nam, Shin, Ochyl, Stuckey, Meagher, Chang, Song, Montefiori, LaBranche, Smith, Xu, Moon: "Lipid-based vaccine nanoparticles for induction of humoral immune responses against HIV-1 and SARS-CoV-2." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 330, pp. 529-539, (2020) (PubMed).

Xiong, Qu, Ciazynska, Hosmillo, Carter, Ebrahimi, Ke, Scheres, Bergamaschi, Grice, Zhang, Nathan, Baker, James, Baxendale, Goodfellow, Doffinger, Briggs: "A thermostable, closed SARS-CoV-2 spike protein trimer." in: Nature structural & molecular biology, Vol. 27, Issue 10, pp. 934-941, (2020) (PubMed).
抗原

SARS-CoV-2 Spike S1

Abstract

SARS-CoV-2 Spike S1 产品

别名

E2 antibody, Surface Glycoprotein antibody, S antibody

物质类

Viral Protein

背景

Protein S (PROS1) is glycoprotein and expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response. Known receptors bind S1 are ACE2, DPP4, CEACAM etc.. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

基因ID

43740568

UniProt

P0DTC2