我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Recombinant SARS-CoV-2 Spike S1 抗体 (RBD)

Name: Recombinant SARS-CoV-2 Spike S1 抗体 (RBD)
Brand: antibodies-online
SKU: ABIN6952546
Availability: OutOfStock
Rating: 5 (1 reviews)

适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma, SARS CoV-2 Eta, SARS CoV-2 Kappa, SARS CoV-2 Delta, SARS CoV-2 Omicron ELISA, IF, Crys, SPR 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody

Multiplex Immunohistochemistry validation image for anti-SARS-CoV-2 Spike S1 (RBD) antibody (ABIN6952546) (Recombinant SARS-CoV-2 Spike S1 抗体 (RBD))

mIHC

Binding curve of four different formats of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)). (Recombinant SARS-CoV-2 Spike S1 抗体 (RBD))

ELISA

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (ABIN6952546) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. (Recombinant SARS-CoV-2 Spike S1 抗体 (RBD))

Surface expression and recognition of minispike by COVID-19 patient serum. (Recombinant SARS-CoV-2 Spike S1 抗体 (RBD))

ISt

VeroE6 cells infected over night with VSVDeltaG-minispike-eGFP (green) are recognized by IgG of S ELISA-positive patients in contrast to a control replicon expressing blue fluorescent protein (VSVDeltaG-BFP). (Recombinant SARS-CoV-2 Spike S1 抗体 (RBD))

ISt

Bind

Neut

17 images

(8 references)

(1 validation)

产品编号 ABIN6952546

发货至: 中国

Datasheet as PDF

Key Features
- Recombinant human neutralizing antibody (hNAb) against SARS-CoV-2.
- Binds SARS-CoV-2 S proteins of lineages B.1.1.7 (alpha), B.1.351 (beta), P.1 (gamma), B.1.617.2 (delta), B.1.1.529 (omicron), B.1.429 (epsilon), B.1.525 (eta), and B.1.617.1 (kappa).
- Frequently used as reference in S-protein ELISAs and neutralization assays.
- Synergizes with other hNAbs: binds a highly conserved epitope, not interefering with the S-protein's ACE2 RBD.
抗原 See all SARS-CoV-2 Spike S1 抗体

SARS-CoV-2 Spike S1

抗体类型

Recombinant Antibody
抗原表位
13

3

2

1
RBD

适用

SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma, SARS CoV-2 Eta, SARS CoV-2 Kappa, SARS CoV-2 Delta, SARS CoV-2 Omicron
宿主
17

9

5

3

2

2

1

1
Human
克隆类型
28

7

5
单克隆
标记
34

3

1

1

1
This SARS-CoV-2 Spike S1 antibody is un-conjugated
应用范围
40

7

5

5

4

4

4

4

4

3

2

1

1
ELISA, Immunofluorescence (IF), Crystallization (Crys), Surface Plasmon Resonance (SPR)

原理

Recombinant monoclonal antibody CR3022 to SARS-CoV S Glycoprotein.

特异性

The antibody CR3022 binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318-510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" conformation of the spike protein (Joyce et al. 2020).
While most S-protein RBD binding antibodies compete for antigen binding with ACE2, the CR3022 epitope does not overlap with the ACE2-binding site. It does thus not hinder binding of neutralizing antibodies. While CR3022 on its own exhibits only a weak neutralizing effect, it has been shown to synergize with other S-protein RBD binding antibodies to neutralize SARS-CoV. This effect still has to be confirmed in context with SARS-CoV-2 (Yuan et al. 2020).

交叉反应（详细）

The anti-SARS-CoV-2 antibody CR3022 was originally discovered in a SARS patient, but it was shown to be a potent binder of SARS-CoV-2 spike protein (S1).

产品特性

Original Species of Ab: Human
Original Format of Ab: IgG1

纯化方法

Protein A affinity purified

免疫原

The original monoclonal antibody was generated through an scFv library derived from a peripheral blood lymphocytes of a patient exposed to the SARS-CoV.

克隆位点

CR3022

亚型

IgG1 kappa
anti-SARS-CoV-2 Spike S1 (RBD) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, Neut 宿主: Human Monoclonal unconjugated Recombinant Antibody

anti-SARS-CoV-2 Spike S1 (RBD) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma, SARS CoV-2 Eta, SARS CoV-2 Kappa ELISA, IF, Crys, SPR 宿主: 兔 Chimeric CR3022 unconjugated Recombinant Antibody

anti-SARS-CoV-2 Spike S1 (RBD) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, Neut, GICA 宿主: Human Monoclonal A1 unconjugated single-domain Antibody (sdAb) Recombinant Antibody

anti-SARS-CoV-2 Spike S1 antibody
适用: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma ELISA, Neut 宿主: Human Chimeric AM122 unconjugated

anti-SARS-CoV-2 Spike S1 antibody
适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, GICA 宿主: Human Chimeric H6 unconjugated Recombinant Antibody

anti-SARS-CoV-2 Spike S1 (RBD) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: 兔 Polyclonal unconjugated

anti-SARS-CoV-2 Spike S1 antibody (Biotin)
适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: Human Monoclonal H6 Biotin scFv fragment Recombinant Antibody

anti-SARS-CoV-2 Spike S1 antibody (HRP)
适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: Human Monoclonal H6 HRP scFv fragment Recombinant Antibody

anti-SARS-CoV-2 Spike S1 antibody
适用: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha ELISA, IHC 宿主: 兔 Monoclonal SP185 unconjugated

anti-SARS-CoV-2 Spike S1 (RBD) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, IF, Crys, SPR 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody

应用备注

This antibody binds to both SARS-CoV and SARS-CoV-2 with high affinity (PMID: 16796401 & 32065055). The initial characterization of the binding of this antibody was performed by ELISA and indicates potential for the development of diagnostic assays, as both virus-capture assays, or as controls in serological assays measuring immune-responses to virus exposure. Human IgG1, IgG3, IgM and IgA isotypes are available to mimic antibody responses seen in COVID19 < a href="https://www.medrxiv.org/content/10.1101/2020.03.17.20037713v1" target="_blank">(Amanat et al. 2020). Human IgG2 and IgG4 subtypes, which are also seen in a small subset of COVID-19 patients, are also available to investigate their role in the response to SARS-CoV-2. The original human IgG1 version of the antibody works synergistically in combination with another non-ompeting SARS antibody CR3014 and is a potential candidate for passive immune prophylaxis of SARS-CoV infection (Meulen et al., 2006). The original antibody (human IgG1) was also reported to bind the 2019-nCoV RBD (KD of 6.3 nM). This antibody has been attributed a potential to be developed as a therapeutic agent, alone or in combination with other neutralizing antibodies for treatment of 2019-nCoV infections (Tian et al., 2020). Bates et al. 2021 (PMID: 32766589) used CR3022 in a immunofluorescence assay.

限制

仅限研究用
生效 #104441 (Multiplex Immunohistochemistry)

by

Akoya Biosciences

No.

#104441

日期

2022.09.13

抗原

SARS-CoV-2 Spike S1

Lot Number

T2023B03

Method validated

Multiplex Immunohistochemistry

Positive Control

FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2

Negative Control

SARS-CoV-2-negative placenta patient sample

Notes

Passed. The anti-SARS-CoV-2 Spike S1 antibody RBD antibody ABIN6952546 produced staining in FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2.

Validation Images

Immunofluorescence of SARS-CoV-2 infection in FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2. Anti-SARS-CoV-2 Spike S1 antibody RBD antibody ABIN6952546 is visualized with PhenoCycler reporters in green and DAPI-stained chromatin in blue.

Immunofluorescence of SARS-CoV-2 infection in FFPE cell pellets from in vitro cultured human lung cells infected with SARS-CoV-2. Costaining with anti-SARS-CoV-2 Spike S1 antibody RBD antibody ABIN6952546 (green) and anti-SARS-CoV-2 Nucleocapsid Protein antibody ABIN6952544 (red).
Full Methods
Primary Antibody

ABIN6952546

Secondary Antibody

Full Protocol

Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual FFPE tissue protocol.
Conjugation of the anti-SARS-CoV-2 Spike S1 antibody RBD antibody ABIN6952546 as described in Chapter 4 of the Akoya Biosciences CODEX® User Manual.
Autofluorescence quenching according to the Autofluorescence Quenching Protocol for CODEX® (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Experimental Notes
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS with 0.02 % Proclin 300.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Kannenberg, Trawinski, Henschler, Buhmann, Hönemann, Jassoy: "Antibody course and memory B-cell response in the first year after SARS-CoV-2 infection." in: The Journal of infectious diseases, (2022) (PubMed).

Jacobsen, Fabricius, Class, Topfstedt, Lorenzetti, Janowska, Schmidt, Staniek, Zernickel, Stamminger, Dietz, Zellmer, Hecht, Rauch, Blum, Ludwig, Jahrsdörfer, Schrezenmeier, Heeg, Mayer, Seidel, Groß et al.: "High antibody levels and reduced cellular response in children up to one year after SARS-CoV-2 infection. ..." in: Nature communications, Vol. 13, Issue 1, pp. 7315, (2022) (PubMed).

Hennrich, Sawatsky, Santos-Mandujano, Banda, Oberhuber, Schopf, Pfaffinger, Wittwer, Riedel, Pfaller, Conzelmann: "Safe and effective two-in-one replicon-and-VLP minispike vaccine for COVID-19: Protection of mice after a single immunization." in: PLoS pathogens, Vol. 17, Issue 4, pp. e1009064, (2021) (PubMed).

Tian, Li, Huang, Xia, Lu, Shi, Lu, Jiang, Yang, Wu, Ying: "Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody." in: Emerging microbes & infections, Vol. 9, Issue 1, pp. 382-385, (2020) (PubMed).

Stadlbauer, Amanat, Chromikova, Jiang, Strohmeier, Arunkumar, Tan, Bhavsar, Capuano, Kirkpatrick, Meade, Brito, Teo, McMahon, Simon, Krammer: "SARS-CoV-2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup." in: Current protocols in microbiology, Vol. 57, Issue 1, pp. e100, (2020) (PubMed).

Yuan, Wu, Zhu, Lee, So, Lv, Mok, Wilson: "A highly conserved cryptic epitope in the receptor-binding domains of SARS-CoV-2 and SARS-CoV." in: Science (New York, N.Y.), (2020) (PubMed).

Pinto, Park, Beltramello, Walls, Tortorici, Bianchi, Jaconi, Culap, Zatta, De Marco, Peter, Guarino, Spreafico, Cameroni, Case, Chen, Havenar-Daughton, Snell, Telenti, Virgin, Lanzavecchia, Diamond et al.: "Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody." in: Nature, (2020) (PubMed).

ter Meulen, van den Brink, Poon, Marissen, Leung, Cox, Cheung, Bakker, Bogaards, van Deventer, Preiser, Doerr, Chow, de Kruif, Peiris, Goudsmit: "Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants." in: PLoS medicine, Vol. 3, Issue 7, pp. e237, (2007) (PubMed).
抗原

SARS-CoV-2 Spike S1

Abstract

SARS-CoV-2 Spike S1 产品

物质类

Viral Protein

背景

Spike protein, COVID19, COVID 19, S protein, SARS-CoV S protein, S glycoprotein, E2, Peplomer protein, Spike protein S1, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV, Ab1680.10, Ab1680.15, Ab1680.16

UniProt

P59594